Microalgae are unicellular organisms which are different colors depending on the pigments in them. Microalgae have the potential for rapid growth compared to plant. Blue green algae usually have a green color and grow autotrophs and facultative. In recent years, microalgae have been widely used in all areas of our lives for scientific purposes such as energy, food and additive source. These studies include the increase of productivity. Various methods such as arrangement of growth parameters and manipulation for increasing high biomass and metabolic content in these areas are frequently tried. In this study, Borodinellopsis texensis 892 was used. It is located in the Chlorophyceae class in green algae. Borodinellopsis texensis 892 was grown in Tris-Acetate-Phosphate (TAP) medium and it was subjected to two-step salinity stress. Firstly, microalgae medium included control, 10, 20, 40, 80 and 160 mM KCI. Then, after stationary phase, microalgal cells were exposed to high salt concentrations (0, 80,160, 320 and 640 mM KCI). In first stage, we observed that biomass concentration increased 1.7 times compared to control. In addition to this, we examined protein and carbohydrate concentrations. After two-step salinity stress, Borodinellopsis texensis 892 had 2.5 times higher carbohydrate yield. In conclusion, Borodinellopsis texensis 892 can be exposed to 40 mM and 640 mM salinity stress for higher biomass and carbohydrate yield, respectively.
Anahtar Kelimeler: Borodinellopsis Texensis, Salinity Stress, Carbohydrate, Biomass